This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
• “What not reading does to your writing.” Lincoln Michel is one of my favorite chroniclers of life as a professional writer, and the point he concludes on here really is the simplest, most effective advice I can give to anyone who wants to write better: “The best way for this author to improve their writing is simple. They should read a few good books.” I notice it in myself. When I am not actively reading fiction, my writing gets more flat.
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